区别就是两者意思上都表示分离，分开，但意思上还有很大的不同，具体不同如下，

pide into中文意思是把……分成

Look at this rough drawing of one of them, one Chromosome about to pide into two.看一下其中一条的草图，一条将要分裂成两条的染色体。

separate with中文意思是与分开

Many parents object to sending their children to study abroad on the account that they don't want to separate with their children.许多父母反对将孩子送去国外留学，原因是他们不想和孩子分开。